Basic principle of strain equipment
The growth of liquid strains must meet the needs of temperature, humidity, oxygen demand, nutrients, etc., while avoiding the infection of miscellaneous bacteria.
In the deep fermentation technology, it is called selective fermentation technology, such as wine production technology is this example,
and white wine production is biological yeast technology. Liquid strain fermentation equipment (including four systems, temperature control system is composed
of controller, electric tube, etc.; The gas supply system is composed of air compressor, conveying pipe, air filter, etc. The cooling system
is composed of heat exchanger and water inlet and outlet pipe. The mixing system is composed of a jet and a lifting tube.
The key technology of strain production
1. The key to the production of liquid bacteria with dissolved oxygen is the amount of oxygen dissolved in the culture medium, because in the process of mycelia growth,
the dissolved oxygen
is constantly absorbed to maintain its own metabolism. The amount of oxygen dissolved in the liquid (water)
is related to pressure and temperature, and also has a great relationship with the contact area and osmotic pressure of the culture medium. Therefore, we effectively solve
these problems
when designing fermentation equipment,
such as the installation of jet to increase the fragmentation of bubbles.
2, the key to air filtration technology is to ensure that the air entering is high sterility, so it is necessary to choose a filter membrane with a small aperture material. Generally,
the diameter of bacteria is 0.5-5UM, yeast is 1-10UM, and viruses are generally 20-400MU, so the above factors should be considered
comprehensively when choosing a filter membrane. Of course, if the selection is too small, the cost will be greatly increased. In addition, the environment has a great impact
on the air, and it is necessary to keep the environment clean in the air compression room and seed production workshop.
3, culture medium Culture medium is the nutrient source of mycelium growth and development, requiring balanced nutrition. Different strains have different nutritional requirements.
The raw materials are sugar, bran, potassium dihydrogen phosphate, magnesium sulfate, vitamin, peptone potato juice,
yeast extract and so on. When configuring the culture medium, first boil the potato slices and bran together, filter out the juice, and then add other auxiliary materials to mix well.
4, the upper end of the inoculation incubator has the inoculation port, which is also the loading port, the mother species and the bottle are added with antibacterial agent,
and then must be poured into the tank under the protection of the flame ring, requiring fast action and accurate operation.
5, environment as mentioned earlier, the environment has a great impact on the production of bacteria, the filter itself can not filter out the miscellaneous bacteria, reducing
the number of miscellaneous bacteria in the environment is very important, the liquid strain mother seed access to
the sterilized culture liquid as long as it can form the growth advantage of miscellaneous bacteria can control the miscellaneous bacteria infection, that is to say,
Liquid culture is not built on the premise of sterility but on the premise of relative sterility. In this sense, keeping the environment clean
and reducing the number of miscellaneous bacteria in the air are of great significance for improving the success rate of bacterial culture.
Model name: strain fermenter
Nominal capacity: 50 l / 100 l / 200 l / 300 l / 500 l / 1000 l / 10000 l
Tank material: all stainless steel 304/316L
Loading coefficient :80%-90%
Mixing method: Flat leaf/oblique leaf/curved leaf/arrow leaf (optional according to customer requirements)
Structure: floor type, American frame structure
Sterilization method: steam sterilization/electric heating sterilization
Ventilation control:manual control, rotameter display. Imported filter membrane air sterilization filter, filtering accuracy up to 0.01MM
Standard interface: temperature control, inoculation port, feed, gas, discharge, PH, dissolved oxygen temperature control: jacket water bath electric heating, automatic control,
temperature control Cooling water temperature +5℃~65℃+0.2℃ feed control :PID automatic setting peristaltic pump switch control,
automatic flow and metering foam control: automatic PID measurement and alarm, Peristaltic pump automatically adds defoamer
Pressure control: manual control (automatic control is optional), instrument display
Mixing control: direct coupled mechanical mixing, top mechanical seal, DC or AC motor, stepless or variable frequency speed regulating air-lift mixing,
mechanical mixing, reducer, inverter speed regulating.
Control mode:
TLFACS automatic fermentation control system, LCD touch screen operation interface, PLC controller. Real time measurement and automatic control of temperature, stirring speed, PH value, DO value, defoaming, multiple feed, acid, alkali, pressure, ventilation and other parameters. With digital setting, quick correction, measurement data display storage and printing, password management. And has a communication interface, can be connected with the host computer. The TL-FACS-M biological process control system can be used to realize more measurement and control functions. Equipment features: tank and pipeline sterilization, safe and reliable. Second gear turbine disc agitator (flat blade/inclined blade/curved blade), height adjustable, with the baffle used to make the mixture of material and liquid more uniform, conducive to gas dispersion and oxygen absorption.
